Differential gene expression analysis by RNA-Seq for the bldC deletion strain and the wild type of Streptomyces venezuelae strain NRRL B-65442 after 10 and 14 hours of culture

The BldC gene is required for the formation of aerial hyphae in Streptomyces venezuelae. It is a 68 amino acid DNA-binding protein related to the MerR family of transcription factors. BldC deletion strains are bald because they initiate sporulation prematurely, omitting the formation of aerial hyphae altogether. This RNA-Seq experiment was carried out to determine and quantify effect of BldC on the expression of all genes in the S. venezuelae genome after 10 and 14 hours of culture.

BldC基因对于Streptomyces venezuelae中气生菌丝的形成至关重要。该基因编码一种由68个氨基酸组成的DNA结合蛋白，与MerR家族的转录因子相关。BldC基因缺失菌株因过早启动孢子形成而缺乏气生菌丝，呈现出秃顶状。为了确定和量化BldC对S. venezuelae基因组中所有基因表达在培养10小时和14小时后的影响，本研究进行了RNA-Seq实验。