Genomic positioning in LBR and Lamin A K562 knockout cells

To probe the functional consequences of nuclear lamina genome association, we made LMNA, LBR, and double LMNA/LBR K562 knockout lines. We first examined the genome interaction changes with nulcear lamina using LMNB1 DamID seq and further assessed the functional consequences of these changes using RNA-seq and repli-seq. LBR and Lamin A genes were disrupted by frame shift mutations induced by CRISPR cutting in K562 cells. In these cells, genome positioning relative to nuclear locales was determined using DamID. Additionally, functional consequences were determined with RNA-seq and Repli-seq. Please note that the processed data file generated from both DamID_LMNB1 and DamID_DamControl sample is linked to the corresponding *DamID_LMNB1 sample records.