DNA metabarcoding parasitoid wasps

We know little about how parasitoids of herbivorous insects use herbivore-induced volatile organic compounds (VOCs) to locate potential hosts on saplings in forests and how this depends on tree composition. Therefore, we performed an experiment in a forest in Poland where we placed pairs of oak saplings (Quercus robur or Q. petraea) in neighbourhoodneighborhoods dominated by oak, beech, or pine trees. We treated one sapling in each pair with the phytohormone methyl jasmonate that triggers induced responses in plants. We measured the VOC emissions of thirty-six saplings and placed Malaise traps with five of the pairs. We counted the parasitoids in the ten Malaise samples and identified them using DNA metabarcoding. The two species of oak differed in both the proportions of VOCs and the specific VOCs that were elevated following the application of methyl jasmonate. We did not detect any overall effects of treatment on parasitoid abundance or community composition. However, some parasitoid species appeared to be attracted to elevated emissions of specific induced VOCs. Parasitoid communities differed significantly between sites and showed marginally significant differences between neighbourhoodneighborhoods. Overall, our results suggest that parasitoids in the understory are affected by tree composition of the canopy, but the effects of VOC emissions were limited.