Quercus spinosa Raw sequence reads

we report a modified method for the isolation of intact chloroplasts, which needs less than 0.5 g leaf material. Coupled with rolling circle amplification (RCA), next-generation sequencing and a pipeline combining de-novo assembly and reference guided assembly (RGA), we successfully obtained a complete chloroplast genome for the non-model forest tree species: evergreen oak Quercus spinosa, with as many as 36% of the sequence reads mapped to the chloroplast genome.