Expression profiling of lung cancer cells reconstituted with SMARCA4 WT and mutant K785R after SMARCA2 knockdown

Using RNA-seq, we identified the expression changes in lung cancer cells (NCI-H1944) transduced with control or SMARCA2-targeting hairpins. We also identified the SMARCA2 program that is rescued by SMARCA4 WT and SMARCA4 K785R transduced. The "SAMPLE_ID" sample characteristic is a sample identifier internal to Genentech. The ID of this project in Genentech's ExpressionPlot database is PRJ0034746 Overall design: NCI-H1944 cells were lentivirally transduced with LACZ, SMARCA4 WT and SMARCA4 K785R and selected with blasticidin for 5 d. Cells were then transduced with nontargeting or SMARCA2-targeting hairpins in triplicate. After 72 h puromycin selection, RNA was isolated from cell pellets.