Supplementary information and datasets from a quantitative stable isotope probing experiment

This dataset contains OTU and taxonomy tables from amplicon sequencing, raw flow cytometry data (.fcs), phylogenetic trees and other supplementary files (Files S1–S5) generated as part of a quantitative stable isotope probing (qSIP) experiment with stream and lake water from lake Siggeforasjön, central Sweden. Amplicon sequencing targeted 16S rRNA (bacteria) and 18S rRNA (protists/eukaryotes) to characterize community composition and calculate taxon-specific excess atom fraction (EAF) isotope incorporation for protist grazers. Flow cytometry quantified bacterial abundance and protist feeding ratios. File S1: Reference sequences for SSU rRNA phylogenies used to refine taxonomy and functional traits (pigmentation, feeding behavior). Files S2–S3: Relative abundances of bacterial phyla and eukaryotic subdivisions for inlet and lake experimental bottles. File S4: Relative abundances of eukaryotic subdivisions across density fractions for qSIP. File S5: Compilation of all qSIP-labeled OTUs with taxonomic, trophic, pigmentation annotations, EAF values and presence across inlet/lake incubations. Phylogenetic trees: Used to refine taxonomic and functional annotations.