Transcriptomic analysis of multipotent-mesenchymal stem cells (M-MSCs) from human pluripotent stem cells (hPSCs)

Mesenchymal stem cells (MSCs) could be one of the candidates for intractable diseases. However, even though adult MSCs could interact with innate and adaptive immune systems and regulate the proliferation, differentiation, and activation of immune cells, they are limited in the in vitro proliferative capacity. For stem cell therapy, MSCs derived from pluripotent stem cells (PSCs) could be another superior option. Multipotent-MSCs (M-MSCs) were generated via the epithelial-mesenchymal transition (EMT) process from human PSCs. The adherent cells from hPSC-derived embryoid bodies were transformed using a commercialized 8-micrometer-porous membrane transwell culture insert in the presence of EMG2-MV media. High levels of typical MSC surface antigen markers were confirmed within the population migrating through the permeable membrane from the upper compartment to the lower compartment. Besides, the population migrating was demonstrated multipotent-differentiation-capable without characteristics and chromosomal changes. The M-MSCs were generated from various humam PSC lines including H9 and SNUhES, and their transcriptomic expressions were analyzed.