Gene expression analysis by RNA-seq on control 5 dpf zebrafish livers

This study was carried out to compare potential changes in the gene expression in 5 dpf livers between untreated, 1.0 and 1.5mM iAs exposures. The samples in this accession include only the 120 hpf untreated, wild-type controls from an experiment comparing the response of zebrafish larval liver to arsenic exposure and to overexpression of the arsenic methyltransferase gene (as3mt) in hepatocytes (tg:(fab10a:as3mt, cry:dsRed). This study was carried out by comparing RNA extracted from 120 hpf zebrafish livers from six groups: untreated or 24-hour exposure to 1 mM or 1.5 mM iAs in CAAX-EGFP positive larvae and CAAX-EGFP positive and as3mt overexpressing larvae.To generate these controls,(tg:(fab10a:as3mt, cry:dsRed) were outcrossed to tg(fabp10a:CAAX-EGFP) and the larvae that only had the CAAX-EGFP expression in the liver were collected. RNA from 12 livers per group collected from 5 clutches (biological replicates) per group were extracted using Trizol/Chlorofom, PolyA selected and sequenced on Illumina NextSeq550.