CC027/GeniUnc mouse strain-derived jejunal epithelial cells before and after peanut sensitization and at 30, 60, and 120 minutes after peanut challenge

Peanut allergy reaction severity correlates with increased intestinal epithelial cell (IEC) barrier permeability. CC027/GeniUnc mice develop peanut allergy by intragastric administration of peanut proteins without adjuvant. We report that peanut-allergic CC027/GeniUnc mice showed increased IEC barrier permeability and systemic peanut allergen Ara h 2 after challenge. Jejunal epithelial cell transcriptomics showed effects of peanut allergy on IEC proliferation, survival, and metabolism, and revealed IEC-predominant angiopoietin like-4 (Angptl4) as a unique feature of CC027/GeniUnc peanut allergy. Peanut-allergic pediatric patients demonstrated significantly higher serum ANGPTL4 compared to non-peanut-allergic but atopic patients, highlighting its potential as a biomarker of peanut allergy. 15 isolated jejunal epithelial cell samples from CC027/GeniUnc mice (n=15) representing 5 experimental conditions: PBS-sensitized & unchallenged (n=3); Peanut-sensitized & unchallenged (n=3); Peanut-sensitized & challenged, 30 minutes post-challenge (n=3); Peanut-sensitized & challenged, 60 minutes post-challenge (n=3); Peanut-sensitized & challenged, 120 minutes post-challenge (n=3).