Transctriptional profiling of murine hematopoietic stem and progenitor cells upon β-glucan administration

Objective: Transcriptional profiling of murine HSPC in response to β-glucan-induced innate immune training HSPC mRNA profiles of wild type (WT) mice injected with PBS or β-glucan. Wild type (WT) C57BL/6 mice were intraperitoneally injected with PBS or 1 mg β-glucan in PBS. Mice were sacrificed on day 7 or day 28 and long-term heematopoietic stem cells (LT-HSC) and/or multipotent progenitors (MPP) were sorted. In another group, mice were injected with PBS or 1 mg β-glucan in PBS and on day 7 they were additionally injected with 150 mg/kg 5-fluouracil. Mice were sacrificed on day 14 after 5-FU administration and LT-HSC were sorted.