Gene expression profile at single cell level of HaCaT cells exposed to ionizing radiation. Gene expression profile at single cell level of HaCaT cells exposed to ionizing radiation

HaCaT cell line is a kind of keratinocyte from human skin. We used single cell RNA sequencing (scRNA-seq) to analyze the influnce of fractionated 2 Gy or single 20 Gy irradiation on HaCaT cells. Overall design: HaCaT cells were administrated with 5 or 10 times fractionated 2 Gy irradiation, and meanwhile at the end of last time fractionated irradiation, a single 20 Gy irradiation was given for cells in another cultrue dish (PF1 for 0 Gy,PF2 for 2 Gy×5,PF3 for 2 Gy×10 and PF4 for 20 Gy). And three days after last time irradiation, cells were collected and analyzed using scRNAseq to investigate the change of cluster profiles.