scRNA-seq of HSC

Human hematopoietic stem/progenitor cells (HSPCs) are enriched in umbilical cord blood (UCB) among cell population that express CD34 and CD133 antigens. These cells can be purified further and sorted by FACS as of CD34+Lin-CD45+ and CD133+Lin-CD45+ cells. It has been postulated that population of CD133+ HSPCs is enriched for more primitive stem cells. To address this issue at the molecular level we performed single-cell RNA-sequencing (scRNA-seq) and analyzed the transcriptome of both cell types. Herein, we report similarities and differences in transcriptome between these cells and, among subpopulations identified by scRNA-seq.