Transcription factor-like 5 is a potential DNA/RNA binding protein essential for maintaining male fertility in mice

Tissue-specific transcriptional factors often play key roles in the development of specific cell lineages. Transcription factor-like 5 (TCFL5) is a testis-specific protein that contains the basic helix-loop-helix domain but the in vivo functions of TCFL5 remained unknown. Here, we generated CRISPR/Cas9-mediated knockout mice to dissect the function of TCFL5 in mouse testes.The target RNAs of TCFL5 in wild-type mouse testes were analyzed by eCLIP-sequencing. The 21d mouse testes were used to performed eCLIP experiment and the purified RNAs by eCLIP were sequenced on the Illumina NovaSeq platform, in two duplicate samples.