C11-BODIPY581/591 staining to investigate pollutants induced lipid peroxidation

The aim of the experiment was to examine the suitability of the dye C11-BODIPY581/591 for the assessment of lipid peroxidation induced by pollutants with different mode of action in the microalga Chlamydomonas reinhardtii
Conclusion:
The dye was successfully applied in a wide range of pollutants and exposure conditions, included studies of lipid peroxidation as a function of time, C11-BODIPY591/581 staining resulted to be a reliable method for studying lipid peroxidation in Chlamydomonas cells Accuri FCM calibration was periodically inspected following the manufacturer instructions during the entire work. Stressor solutions were all purity grade and algal axenicity was repeatedly checked during the period of experiments. All experiments were performed at least three times as independent replicates