Characterizing the gene expression profile of Prox1+ intestinal adenoma organoid cells. Characterizing the gene expression profile of Prox1+ intestinal adenoma organoid cells

We isolated and selected intestinal adenoma organoids from Apcmin/+; Rosa26LSL-TdTomato; Prox1-CreERT2 mice. After the selection procedure without growth factors, we induced CreERT2 activity and the transcription of tdTomato to label Prox1+ cells by 300 nM 4-hydroxytamoxifen for 16h. tdTomato+ (Prox1+) and tdTomato- cells (enriched for Prox1- cells) were FACS sorted and total RNA was isolated. Overall design: Total RNA obtained from tdTomato+ (Prox1+) cells was compared to the tdTomato- cell population (enriched for Prox1- cells) after FACS sorting.