Doxorubicin Changes the Spatial Organization of the Genome around Active Promoters. Doxorubicin Changes the Spatial Organization of the Genome around Active Promoters

To investigate the function of TOP2 on chromatin structure of human cells, we performed Hi-C using chromatin obtained from RPE1 cells. Overall design: Pairwise comparison of the Hi-C maps of four different conditions: 1) control (DMSO-treatment), 2) treated with ICRF193 in final concentration 5uM or treated with doxorubicin in final concentration 3) 200 nM and 4) 340 nM.