Methylation profiling of cold exposure induced browning of white fat

We set two groups of 2-month-old C57BL6/J male mice at either thermoneutral temperature of 30? or under cold stimulation at 4? for one week. To identify events vital to beige fat activation, we performed MeRIP-seq using mice subcutaneous adipose tissue (iWAT) and focused on differentially-expressed genes characterized significant upregulation. Overall design: The biege fat obtained from the mice with housed at either 30? or 4? for 7 days in a temperature-controlled incubator