Supplementary datasets for: Large-scale genome sequencing reveals the driving forces of viruses in microalgal evolution

Microalgae are integral primary producers for global ecosystems whose genomes can be mined for ecological insights, but representative genome sequences are lacking for many phyla. We cultured and sequenced 107 microalgae species from 11 different phyla indigenous to varied geographies and climates. This genome collection was used to resolve genomic differences between saltwater and freshwater microalgae. Freshwater species showed domain-centric ontology enrichment for nuclear and nuclear membrane functions, while saltwater species were enriched in organellar and cellular membrane functions. Marine species contained significantly more viral families in their genomes (p-value = 8 x 10(-4)). Viral sequences were identified from Chlorovirus, Coccolithovirus, Pandoravirus, Marseillevirus, Tupanvirus, and others integrated into algal genomes. Algal, viral-origin sequences were found to be expressed and to code for a wide variety of functions. Our results clarify the poorly characterized occur...