Small RNAseq analysis of C.elegans from Parental P0 to F3 generations under hypoxic stress in both WT and hrde-1 mutant [small RNA-seq]

The goal of small RNA-seq is to identify the differentially expressed small RNAs (microRNA, 22G RNA, piRNA, snoRNA) in the wild-type (WT) and hrde-1 mutant of C.elegans at the normoxia reared and hypoxia treatment conditions at P0 generation, and those small RNAs maintained dysregulation in F1, F2 and F3 generation. Total RNA from WT and hrde-1 under normoxia or hypoxia treatment worms from P0 to F3 generations were extacted, and 32 small RNA libraries were built using the Nextflex Small RNA-seq Kit v3 (NOVA-5132-06), in duplicate for each condition, using single-end 85 bp on Illumina Illumina Nextseq 500 platform