IL-10-expressing CAR T cells resist dysfunction and mediate durable clearance of solid tumors and metastases

We used single-cell sequencing to compare gene expression and cell subset distribution of conventional CAR-T cells and IL-10-expressing CAR-T cells in the spleen and tumors. Overall design: In a murine tumor model, we sorted HER2 CAR-T cells and IL-10 HER2 CAR-T cells in tumors and spleens at day 18 post-tumor inoculation. The groups were as follows: G1 - HER2 CAR-T cells in tumors, G2 - IL-10 expressing HER2 CAR-T cells in tumors, and G3 - IL-10 expressing HER2 CAR-T cells in the spleen.