RNA sequencing of porcine smooth muscle cells, after treatments with platelet derived growth factor and S100A4 protein

Smooth muscle cells were treated with platelet derived growth factor (PDGF-BB) and S100A4 protein to decipher the mechanisms by which they contribute to smooth muscle cell phenotypic transition. We report that PDGF-BB treatment upregulates genes related to growth response or to extracellular matrix component proteases, while S100A4 upregulates pro-inflammatory genes. When used in combination, PDGF-BB and S100A4 show synergistic action by enhancing the upregulation of genes already affected by S100A4 and by inducing the upregulation of genes exclusive for this condition. Cells were exposed to different treatments (i.e. PDGF-BB, oS100A4 and PDGF-BB+oS100A4) for 4 hours. Experiments were performed with cells isolated from 3 different animals.