Fine-Tuning of PGC1a Expression Regulates Cardiac Function and Longevity (RNA-seq: WT vs PKI)

PGC1alpha plays an important role in energy metabolism and mitochondrial function in the heart, multiple attempts have failed to upregulate PGC1alpha expression as a therapy, with the underlying mechanism to be answered. We used RNA sequencing to detail the global programme of gene expression in the hearts of wild-type mice with or without cardiomyocyte-restricted PGC1alpha overexpression. Overall design: Heart mRNA profiles of 12-month old wild-type (WT) and PGC1alpha overexpressed (PKI) mice were generated by deep sequencing, using Illumina HiSeq.