Eif3d safeguards the homeostasis of Key signaling pathways in Human primed pluripotency

Although pluripotent stem cell (PSC) properties, such as differentiation and infinite proliferation, have been well documented within the frameworks of transcription factor networks, epigenomes, and signal transduction, they remain unclear and fragmented. Directing attention toward translational regulation as a bridge between these events can yield additional insights into previously unexplained mechanisms. Our functional CRISPR interference screen-based approach revealed that EIF3D, a translation initiation factor, is crucial for maintaining primed pluripotency. Loss of EIF3D disrupted the balance of pluripotency-associated signaling pathways, thereby compromising primed pluripotency. Moreover, EIF3D ensured robust proliferation by controlling the translation of various p53 regulators, which maintain low p53 activity in the undifferentiated state. In this way, EIF3D-mediated translation contributes to tuning the homeostasis of the primed pluripotency networks, ensuring the maintenance ..., These are raw data, uncropped images, numerical data, and statistical results from the manuscript \"EIF3D Safeguards the Homeostasis of Key Signaling Pathways in Human Primed Pluripotency\" by Okubo et al., , # Eif3d safeguards the homeostasis of Key signaling pathways in Human primed pluripotency

[https://doi.org/10.5061/dryad.kkwh70sb6](https://doi.org/10.5061/dryad.kkwh70sb6)

This data set contains the original data used in the paper \"Eif3d safeguards the homeostasis of key signaling pathways in Human primed pluripotency\" published in Science Advances. The .fcs files are the raw data for flow cytometry analysis, the .tif files are the raw image data for immune-stained cells, the .pdf files are the raw data for Western blotting, and the .xls file contains the numerical data and statistical test results used in the paper.

## Description of the dataset

### 20221114\_3D1-1\_control.fcs

* The flow cytometry data of EIF3D knockdown human iPS cel clone 1-1 stained for DNA.

### 20221114\_3D1-1.fcs

* The flow cytometry data of EIF3D knockdown human iPS cell clone 1-1 stained for EdU (5-ethynyl-2�f-deoxyuridine) and DNA.

### 20221114\_3D1-1(EdU-).fcs

* The flow cytometry data of EIF3D knoc...,