Table 2_Genome-wide sRNA and mRNA transcriptomic profiling insights into carbapenem-resistant Acinetobacter baumannii.xlsx

IntroductionAcinetobacter baumannii (AB) is rising as a human pathogen of critical priority worldwide as it is the leading cause of opportunistic infections in healthcare settings and carbapenem-resistant AB is listed as a “super bacterium” or “priority pathogen for drug resistance” by the World Health Organization.MethodsClinical isolates of A. baumannii were collected and tested for antimicrobial susceptibility. Among them, carbapenem-resistant and carbapenem-sensitive A. baumannii were subjected to prokaryotic transcriptome sequencing. The change of sRNA and mRNA expression was analyzed by bioinformatics and validated by quantitative reverse transcription-PCR.ResultsA total of 687 clinical isolates were collected, of which 336 strains of A. baumannii were resistant to carbapenem. Five hundred and six differentially expressed genes and nineteen differentially expressed sRNA candidates were discovered through transcriptomic profile analysis between carbapenem-resistant isolates and carbapenem-sensitive isolates. Possible binding sites were predicted through software for sRNA21 and adeK, sRNA27 and pgaC, sRNA29 and adeB, sRNA36 and katG, indicating a possible targeting relationship. A negative correlation was shown between sRNA21 and adeK (r = -0.581, P = 0.007), sRNA27 and pgaC (r = -0.612, P = 0.004), sRNA29 and adeB (r = -0.516, P = 0.020).DiscussionThis study preliminarily screened differentially expressed mRNA and sRNA in carbapenem-resistant A. baumannii, and explored possible targeting relationships, which will help further reveal the resistance mechanism and provide a theoretical basis for the development of drugs targeting sRNA for the prevention and treatment of carbapenem-resistant A. baumannii infection.

介绍丙酸杆菌鲍曼氏菌（AB）作为全球范围内人类病原体的地位日益上升，已成为医疗环境中机会性感染的首要原因。耐药性碳青霉烯类丙酸杆菌被世界卫生组织列为“超级细菌”或“耐药性优先病原体”。方法：收集并测试了丙酸杆菌鲍曼氏菌的临床分离株对抗菌药物的敏感性。其中，耐药性和敏感性的丙酸杆菌鲍曼氏菌均接受了原核转录组测序。通过生物信息学分析sRNA和mRNA表达的变化，并通过定量逆转录PCR进行验证。结果：共收集了687个临床分离株，其中336株丙酸杆菌鲍曼氏菌对碳青霉烯类抗生素具有耐药性。通过碳青霉烯类耐药分离株与敏感分离株之间的转录组分析，发现了506个差异表达基因和19个差异表达sRNA候选基因。通过sRNA21和adeK、sRNA27和pgaC、sRNA29和adeB、sRNA36和katG的软件预测可能结合位点，表明可能存在靶向关系。sRNA21与adeK（r = -0.581，P = 0.007）、sRNA27与pgaC（r = -0.612，P = 0.004）、sRNA29与adeB（r = -0.516，P = 0.020）之间显示出负相关。讨论：本研究初步筛选了碳青霉烯类耐药性丙酸杆菌鲍曼氏菌中的差异表达mRNA和sRNA，并探讨了可能的靶向关系，这将有助于进一步揭示耐药机制，并为开发针对sRNA的药物提供理论基础，以预防和治疗碳青霉烯类耐药性丙酸杆菌鲍曼氏菌感染。