Validation of STAT5-dependent, IL-2-mediated gene expression changes.

Transcript level changes of 57 intersect genes were measured using qRT2PCR arrays in PHA activated, quiescent and IL-2 stimulated (3 and 6 h) PBMCs (72 h activated, 48 h quiescent, from 3 independent donors). Fold response and the p-value to the un-stimulated control samples are shown. Bold letters indicate significantly up-regulated, while italic letters represent significantly down-regulated genes. Stars specify known target genes. Genomic locations are marked as follows: Pp, Proximal Promoter; ID, Immediate Downstream; En, Enhancer; E, Exon. Unlabeled boxes contain genes that were identified by GEA but not chip-on-ChIP.