Water availability and temperature as modifiers of evaporative water loss in tropical frogs

Water plays a notable role in the ecology of most terrestrial organisms due to the risks associated with water loss. Specifically, water loss in terrestrial animals happens through evaporation across respiratory tissues or epidermis. Amphibians are ideal systems for studying how abiotic factors impact water loss since their bodies often respond quickly to environmental changes. While the effect of temperature on water loss is well known across many taxa, we are still learning how temperature in combination with humidity or water availability affects water loss. Here, we tested how standing water sources (availability) and temperature (26 and 36°C) together affect water loss in anuran amphibians using a Bayesian framework. We also present a conceptual model for considering how water availability and temperature may interact, resulting in body mass changes. After accounting for phylogenetic and time autocorrelation, we determined how different variables (water loss and uptake rates, tempe..., The dataset was collected at Stanford University as described in doi.org/10.1093/icb/icae057. The dataset contains body mass data corresponding to frogs exposed to various treatments, along with associated metadata. The dataset was processed by imputing some missing values, fitting a longitudinal phylogenetic linear mixed model in a Bayesian framework, and plotting the raw data, results, and diagnostics. , , # Data and code for Water availability and temperature as modifiers of evaporative water loss in tropical frogs

This repository contains 12 files total. The dataset contains 3 raw data and metadata files (amph_shl_new_Consensus_7238.tre, trees.zip, and raw_dehydration.csv.zip, raw_dehydration_METADATA.txt), 2 analysis files (analysis_github.R, model_github.stan), and 6 files for plotting data and results (fit.RDS.zip, res.RData, write_up12github.qmd, plots_github.R, Fig_1.R, plot_data.R). 

## Description of the data and file structure

The tree files (.tre and .nex within trees.zip) were obtained from [https://vertlife.org/phylosubsets/](https://vertlife.org/phylosubsets/). raw_dehydration.csv.zip contains newly generated data. The two analysis files output the model results and associated diagnostics, summaries, and plots (two exceptions to this are found at the end of this section). fit.RDS.zip contains the posterior distributions resulting from the Bayesian analysis, res.RData is a...