Raman microscopy of live Saccharomyces cerevisiae yeast

We acquired coherent Raman microscopy data on budding yeast Saccharomyces cerevisiae (wild-type) and two mutants, aah1∆ and rad6∆. Our aim was to collect spatially resolved biochemical data on living yeast cells. The image preview (rgb_overlay_BY4741_WT.png) is a composite of three single-wavenumber SRS acquisitions, taken at 1,650 (red), 2,850 (green) and 2,937 cm−1 (blue). This record contains the raw LIF files and metadata in JSON and CSV format. This release accompanies the pub "Coherent Raman imaging of live, unfixed Saccharomyces cerevisiae and mutants aah1∆ and rad6∆", and the GitHub repository for visualizing the dataset and exporting images and metadata is here.