Identification of genes regulated by the MADS transcription factor (TF), SEPALLATA3 spliced variant, SEP3.3 , in the context of the triple sep1 sep2 sep3 mutant background, by RNA-Seq analysis

These analyses allowed the identification of more than 2000 genes regulated by the MADS TF, SEPALLATA3.3 spliced variant(renamed SEP3Δtet or SEP3deltet). RNA seq analysis were performed on inflorescence meristem and flower buds up to stage 10-11 harvested from Arabidopsis plants grown in parralel in long days conditions. Experiments were performed on 3 independant lines sep1sep2sep3 expressing SEP3Δtet . These lines are described in Hugouvieux et al, Nucleic Acid Research, 2018:1;46(10):4966-4977. doi: 10.1093/nar/gky205. Libraries and sequencing were performed by Genewiz (Illumina High SEq, 2X150 bp configuration).