BBB permeability, sleep and qRT-PCR data for: The Drosophila blood-brain barrier regulates sleep via moody GPCR signaling

Sleep is vital for most animals, yet its mechanism and function remain unclear. We found that permeability of the BBB–the organ required for maintenance of homeostatic levels of nutrients, ions, and other molecules in the brain–is modulated by sleep deprivation and can cell-autonomously effect sleep changes. We observed increased BBB permeability in known sleep mutants as well as in acutely sleep deprived animals. In addition to molecular tracers, sleep deprivation-induced BBB changes also increased penetration of drugs used in the treatment of brain pathologies. After chronic/ genetic or acute sleep deprivation, rebound sleep or administration of the sleeping aid gaboxadol normalized BBB permeability, showing that sleep deprivation effects on the BBB are reversible. Along with BBB permeability, RNA levels of the BBB master regulator moody are modulated by sleep. Conversely, altering BBB permeability alone through glia-specific modulation of moody, gαo, loco, lachesin, or neuroglian – e..., Fly genetics
Flies were raised on standard cornmeal/molasses food at 25°C in a 12-hour light/12-hour dark (LD) cycle. Wildtype strains used as controls were isogenic w1118 (iso1CJ) strains (107, 108) for sleep. ln all experiments, Gal4 and UAS parental controls were tested as hemizygotes. The following fly stocks were used: redeye (rye, Bloomington #80692) or nicotinic Acetylcholine Receptor α4 (61), shaker5 (sh, Bloomington #111), hyperkinetic2 (hk, Bloomington #55), fumin (fmn, was a lab stock), sleeplessP1(sss, gift from A. Sehgal),   insomniac1 (inc), and wide awakeD1 (wakeD1) and wide awakeD2 (wakeD2, gifts from Mark Wu), moody-Gal4 (44); UAS-loco (Schwabe et al., 2005); GaO-RNAi #3, and repo-GeneSwitch were gifts from U. Gaul, TH-Gal4 was a lab stocks; Tdc2-Gal4 and UAS-dTRPA1 are from the Bloomington stock center (#9313 and #26264, respectively). nrgRNAi, GaO-RNAi#1, #2 and #3, lachesin-RNAi, pkaC1-RNAi, moodyRNAi are VDRC #9248, #28201gd, #19124, #28010, #28940, #31277, #36821, ..., , # BBB permeability, sleep and qRT-PCR data for \"The *Drosophila* blood-brain barrier regulates sleep via *moody* G-protein coupled receptor signaling\"

[https://doi.org/10.5061/dryad.15dv41p39](https://doi.org/10.5061/dryad.15dv41p39)
Shown are:
For Figures 1D, E, H, I, K, L, 2B, D, F, 3B, C, F, G, 4C, D, H, I, S1C,D, S2C, S3D, E, F, G: 12 or 24 hour sleep and blood-brain barrier (BBB) permeability data for individual flies.
For Figure 3I: Individual quantitative real-time polymerase chain reaction (qRT-PCR) results from 3 separate experiments

## Description of the data and file structure

Tab 1: Sleep and BBB data for Figs 1,2,3: Thermogenetic sleep deprivation, mechanical sleep deprivation, drug penetration into the brain
Tab 2: Sleep and BBB permeability data for sleep mutants in Figure 3.
Tab 3: qRT-PCR data for *moody* RNA in wt, sleep deprivation (SD) and different sleep mutants
Tab 4: BBB permeability and sleep parameter data for Supplemental figures
BBB permeability units are [...