Ifh1 activates RP genes

Experiment Design: __Type of experiment: time course after GAL (galactose) induction __Experimental factor: time, genotype __Number of hybridizations performed in the experiment: 24 (eight conditions with three biological replicates each) Samples used, extract preparation and labeling: __The origin of the biological sample: Species: S. cerevisiae Cell type: wild-type (YSS37, IFH1-13myc-TRP1, MATa) UASIFH1::HIS3-UASGAL1 (YSS89, UASGAL1-IFH1-13myc-TRP1, MATa) __Growth conditions: log phase (2x107/mL) at 30_C in YPLG medium, then addition of Galactose to 2% __Treatments: all cells were harvested by rapid centrifugation at 4_C then flash frozen in liquid nitrogen.实验设计：__实验类型：半乳糖（galactose）诱导后的时间进程实验 __实验因素：时间、基因型 __本实验完成的杂交总数：24组（8种实验条件各设置3次生物学重复） 所用样本、提取物制备与标记：__生物样本来源：物种：酿酒酵母（S. cerevisiae） 细胞类型：野生型菌株（YSS37, IFH1-13myc-TRP1, MATa）；UASIFH1::HIS3-UASGAL1菌株（YSS89, UASGAL1-IFH1-13myc-TRP1, MATa） __培养条件：于30℃下在YPLG培养基中培养至对数生长期（细胞密度达2×10^7个/mL），随后添加半乳糖至终浓度为2% __处理方式：所有细胞均通过4℃快速离心收集，随后置于液氮中快速冷冻。