The simple light isotope metabolic-labeling technique relies on the in-vivo biosynthesis of amino acids from U-[12C]-labeled molecules provided as the sole carbon source. The incorporation of the resu

NIAID Data Ecosystem00

Parsing and Quantification of Raw Orbitrap Mass Spectrometer Data Using RawQuant

质谱数据分析

定量蛋白质组学

Effective analysis of protein samples by mass spectrometry (MS) requires careful selection and optimization of a range of experimental parameters. As the output from the primary detection device, the

Figshare2018-04-26 更新00

The paired primers used for gene cloning, disruption, complement, silencing and expression.

基因功能研究

分子克隆

*H = A, C or T; B = C, G or T; V = A, C or G; R = A or G. The enzyme sites are italicized while the gateway fragments for exchange are in bold.

Figshare2015-12-02 更新00

Quantitative proteomics of cells treated with decaged WZB117-PPG

定量蛋白质组学

细胞药物响应机制

To further understand how cells response to decaged WZB117-PPG, we performed quantitative proteomics of A549 cells under WZB117-PPG treatment (30 μM) with illumination and normal condition by TMT 10 p

NIAID Data Ecosystem00

TMT 6-plex proteome analyisis of USP8-depleted cells

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