Spore display of processive alginate modifying enzymes

Content of the data folders overview.

"Results_and_calc" folder: Contains excel-sheets with processed data results and calculations

    TEV.xlsx: Excel sheet with 1) integrals and calculations of product fractions, and 2) comparison of conditions and error calc    Recycling_50C.xlsx: Excell sheet with integrals and calculations for the 50C recycling experiment    Recycling_37C.xlsx: Excell sheet with integrals and calculations for the 37C recycling experiment    24h_test.xlsx: Integrals and calculations for all strain experiments run for 24h    AlgE4_spore_TR_int.txt: Integrals and time point for the time-resolved spectra    Fluorescence_GFP_strain_comp.xlsx: Fluorescence measurements and calculations for comparison of different sfGFP strains for TEV cleavage    Fluorescence_GFP_conditions_comp.xlsx: Fluorescence measurements and calculations for comparison of different TEV cleavage conditions (Spore OD, BSA conc, TEV conc)    

"Spore_TEV_nov24" folder: Contains NMR spectra for TEV cleavage experiment

    Experiment title: Strain number. Condition. Date. Solvent    "+SN" = TEV added, reaction performed by supernatant    "-SN" = no TEV added, reaction performed by supernatant    "+Spore" = TEV added, reaction performed by spore    "-Spore" = no TEV added, reaction performed by spore

"Spore_okt" folder: Contains NMR spectra for spore recycling experiment at 37C, and seven 24h test of strains

    Samples named: "Recycling" or "Spore recycling" refer to the recycling experiment.        The sample ID refers to the reaction step number (1-5) and a letter showing which of the triplicates it is (A-C)    Samples named: "BS29XXXX 24h test" show one of the triplicates of a 24h activity test of the strain mentioned

"Spore_epi_oct24" folder: Contains NMR spectra for 24h test of all strains    Experiment title shows: Strain number, and an ID for which of the triplicates they are (A-C)        For strains where only B and C are present, the A run of the triplicate is located in fodler "Spore_okt"

"Alginate_recycling20240906" folder: Contains NMR spectra for spore recycling experiment at 50C.

    Experiment title shows: strain number, sample ID, and a description of reaction conditions.        The sample ID refers to the reaction step number (1-5) and a letter showing which of the triplicates it is (A-C)

"Time_resolved" folder: Contains four NMR spectra

    696: 1D 1H spectra without water suppression for water signal calibration    697: 1D 13C spectra for check of setup    698: pseudo-2D 13C experiment. Timeresolved spectrum. Spores were added, and sample was shimmed again right before start of this experiment    699: 1H-13C HSQC to assign products formed