Single-cell RNA sequencing reveals a role of Phf2 in promoting the exit of postnatal neural stem cell from shallow quiescence.

Dentate gyrus single cell mRNA profiles of 30-day-old wild-type (WT) and Phf2 conditional knockout (Phf2-/-) mice were generated by single cell sequencing. We performed data filtering: i) for the genes expressed in at least 30 cells and cells with detected expression of at least 200 genes and no more than 8000 genes; ii) cells with the percentage of expressed mitochondrial genes no more than 10%. Potential cell doublets were filtered out using scrublet (https://pypi.org/project/scrublet/). Single cell clustering was done using scanpy package (https://scanpy.readthedocs.io/en/stable/). Single cell mRNA profiles of 30-day old Nestin-Cre Tg wild type (Phf2+/+) control mice and conditional KO (Nestin-Cre Tg, Phf2flox/flox) mutant mice.本数据集针对30日龄野生型（wild-type，WT）与Phf2条件性敲除（conditional knockout，Phf2-/-）小鼠的齿状回（Dentate gyrus）组织，通过单细胞测序（single cell sequencing）技术获取了其单细胞mRNA表达谱。我们执行了如下数据过滤流程：i）仅保留在至少30个细胞中存在表达的基因，以及检测到至少200个且不超过8000个表达基因的细胞；ii）过滤线粒体基因表达占比超过10%的细胞。随后使用scrublet工具（https://pypi.org/project/scrublet/）去除潜在的细胞双胞体（cell doublets）。单细胞聚类分析通过scanpy包（https://scanpy.readthedocs.io/en/stable/）完成。本数据集同时涵盖30日龄Nestin-Cre Tg野生型（Phf2+/+）对照小鼠，以及条件性敲除突变小鼠（Nestin-Cre Tg，Phf2flox/flox）的单细胞mRNA表达谱。