Additional file 1 of Large-scale morphometry of the subarachnoid space of the optic nerve

Additional file 1: Figure S1. Fixation-generated spurious artifacts in the grayscale slices, featuring strong intensity gradients and creating manifolds in the CSF (left). Issue mitigation during image enhancement and image segmentation (right) of CSF (red). Figure S2. High-angle views from within the grayscale images (intensities mapped from translucent blue to opaque red) showing the multi-scale features of the ON SAS microstructure. Figure S3. Image cuts in the z-direction (top) and x-direction (bottom) of Dataset1. Extended CSF (green) is obtained by engulfing the ON SAS microstructure through a morphological closing operation. Figure S4. Circular sectors (red) in Dataset1 (top) and Dataset2 (bottom) where CSF volume and ON SAS area have been ignored due to the structural deformation occurred during the manipulation of the specimen. Movie S5. Slice-by-slice movies of trabecular thickness and trabecular separation of the ON SAS foreground, both for Dataset1 and Dataset2. Blue corresponds to 2μm or less and red corresponds to 160μm. Movie S6. Overview movies of 3D rendering of opaque isosurfaces of trabecular thickness and trabecular separation at the ON SAS foreground/background interface, both for Dataset1 and Dataset2. Blue corresponds to 2μm or less and red corresponds to 160μm. Movie S7. Closeup movies showing detailed morphometry of the microstructure in the ON SAS, both for Dataset1 and Dataset2. Blue corresponds to 2μm or less and red corresponds to 160μm. Movie S8. Slice-by-slice movies of the stitched signals for both datasets, alongside with image segmentation of the ON SAS trabeculae (yellow) and CSF (red). Movie S9. Volume-rendered close-up of the stitched signal of Dataset1, following the CSF over the ON section.