Daily nitrogen budget, off-gas N2 closure, EPS composition, and 16S community tables for biochar-dosed anammox SBBRs (days 1–95)

This dataset contains the experimental measurements and derived performance indicators from a 95-day anoxic sequencing batch biofilm reactor (AnSBBR) study evaluating the effect of corncob biochar powder dose on anammox nitrogen removal, nitrate-yield suppression, and nitrogen-removal pathway verification via gas/liquid closure. Four reactors were operated at 32 ± 2°C using polyurethane sponge carriers (50% v/v): R0 (0 g biochar), R1 (0.02 g), R2 (0.2 g), and R3 (2.0 g).
The dataset includes daily influent/effluent NH₄⁺-N, NO₂⁻-N, NO₃⁻-N, calculated total inorganic nitrogen (TIN), removal efficiencies (ARE, TNRE), daily nitrogen loading/removal terms (Nin, Nout, Nrem), volumetric nitrogen removal rate (NRR), stoichiometric diagnostics (ΔNO₂/ΔNH₄, NO₃/ΔNH₄, and a nitrate yield proxy), and daily off-gas N₂–N estimates from measured off-gas flow and N₂ fraction with computed closure ratio and N₂/Nrem agreement.
To support mechanistic interpretation, the dataset further includes EPS fractionation results (S-EPS, LB-EPS, TB-EPS) with protein/polysaccharide quantification and PN/PS indices, alongside processed 3D-EEM fluorescence outputs. Biofilm samples collected on days 60, 80, and 95 were also subjected to 16S rRNA gene amplicon sequencing; you may optionally include processed community tables and sample metadata to reproduce Bray–Curtis/PCoA/PERMANOVA/RDA analyses reported in the study and link to any external sequence-read accession used in the manuscript.