Suppl. Information to "The tropical coral Pocillopora acuta displays an unusual chromatin structure and shows histone H3 clipping plasticity upon bleaching"

Supplementary File 1: Multiple alignment for protein sequences of core histones with Pocillopora acuta, Pocillopora damicornis, Acropora digitifera, Nematostella vectensis, Hydra vulgaris, Schistosoma mansoni and Mus musculus. A. Histone H2A; B. Histone H2B; C. Histone H3; D. Histone H4. An asterisk (*) means that the amino acid is conserved between all species.

Supplementary File 2: Original (uncropped and unedited) images used for Figures 1 to 4.

Supplementary data 3: P. acuta nuclei and Symbiodinium count on a Thoma cell counting chamber done over three different nuclei extractions. For each extraction, two counts were performed. P. acuta nuclei were stained with Hoechst 33342 and display a blue fluorescence at 350 nm. Symbiodinium are not damaged by our extraction method and are not permeable to Hoechst. They display a red fluorescence because of their chlorophyl content. Observations were done on a Leica DMLB with objective PL Fluotar 40x and 100x.

Extraction #1 replicate 1: 102 P. acuta nuclei (Blue) ; 2 Symbiodinium (Red)
Extraction #1 replicate 2: 112 P. acuta nuclei (Blue) ; 2 Symbiodinium (Red)

Extraction #1 replicate 1: 42 P. acuta nuclei (Blue) ; 0 Symbiodinium (Red)
Extraction #1 replicate 2: 55 P. acuta nuclei (Blue) ; 1 Symbiodinium (Red)

Extraction #1 replicate 1: 215 P. acuta nuclei (Blue) ; 3 Symbiodinium (Red)
Extraction #1 replicate 2: 257 P. acuta nuclei (Blue) ; 5 Symbiodinium (Red)

Made at IHPE.