Transcriptomic analysis of the biocontrol fungus Trichoderma atroviride overgrowing Verticilluim dahliae provides the identification of mycoparasitic candidate genes aganinst this plant pathogenic fungus

A Trichoderma microarrays composed of 385,000 probes, designed against the genomes of Trichoderma reesei (= Hypocrea jecorina), ID: 431241 (9,129 genes) + Trichoderma virens (= Hypocrea virens), ID: 413071 (11,643 genes) + Trichoderma atroviride (= Hypocrea atroviridis) ID: 197014A (11,643 genes), was constructed (Roche-NimbleGen, Inc., Madison, WI, USA). Probes contained entere transcript sequence. This microarray was used to analyze the transcriptomic changes of T. atroviride IMI 352941 (T11) in three conditions: T11 growing alone, T11 growing at ca 5 mm of V. dahliae V-138I and T11 overgrowing V-138I. Three samples were analyzed as follows: T. atroviride IMI 352941 (T11) grown alone (SOM05CVT), used as control, T11 grown at ca. 5 mm of V. dahliae V-138I (SOM05CVV) and T11 overgrowing V-138I (SOM05CVW). Three replicates for each sample were performed.