Effect of a high fat diet on gene expression in enteroendocrine cells of the mouse jejunum

To identify the genes differentially expressed in jejunal enteroendocrine cells (EECs) under a high fat diet we purified EEC (eYFP+) and non-EEC(eYFP-) using FACS from jejunal villi of Neurog3eYFP/+ mice (Mellitzer et al, Mol. Endo 2004) fed with a normal chow or high fat diet (HFD). In Neurog3eYFP/+ mice enteroendocrine progenitors and their descendants are labeled with eYFP. We then purified the RNA and perfomed gene exspression profiling using RNAseq. All samples are from jejunal villi: Normal diet (ND), EEC (eYFP-plus) (n=3); non-EEC (eYFP-minus) (n=3); High fat diet (HFD) EEC (eYFP-plus) (n=3), non-EEC (eYFP-minus) (n=3).