16s rRNA sequencing to assess the effect of 2H-GPS on gut microbiota composition of Alzheimer's mouse model

In our previous study, we found that gentiopicroside isolated from Gentiana rigescens Franch has a significant antiaging activity and regulates mitophagy and oxidative stress. On the basis of these studies, several compounds were synthesized and evaluated for neuroprotective activity with PC12 cells bioassay and yeast lifespan assay, and 2Hgentiopicroside was selected for further study. The result in this study show that 2HGPS improves the symptoms of AD mice through combination of the regulation of Wnt signaling pathway and the microbiota gut brain axis, and the mechanism of action of 2H-GPS is distinct from that of Done.