Data and code for "Biased Connectivity Shapes Food Odour Categorisation in the Drosophila Mushroom Body"

Overview This dataset contains in vivo calcium-imaging data from adult Drosophila melanogaster and custom analysis code developed for the study “Biased Connectivity Shapes Food Odour Categorisation in the Drosophila Mushroom Body.” The study investigated whether structured connectivity between the olfactory projection neurons and the mushroom body Kenyon cells contributes to the categorisation of food and non-food odours in the mushroom body. Deposited files The deposited files include: - 0README.md: documentation of the deposited data, code, file structure, processing workflow, and software requirements; - calcium_imaging_data_tar.xz: in vivo calcium-imaging data from all flies included in the publication and the registration parameters; - calcium_imaging_data.sha256: checksums for the calcium-imaging data. - traces.tar.xz: calcium traces obtained after image registration; - code.tar.xz: custom code used to analyse the data and generate the figures in the publication; - BiasedConnOdourCat.sif: an Apptainer/Singularity container providing the software environment required to run the code. - python_licenses.md: licenses for the container Python modules. - container_licenses.md: licenses for the container Debian packages. Data acquisition and processing In vivo calcium-imaging data were acquired from five flies for each Kenyon cell type examined in the study. Odour-evoked neural activity was recorded using a two-photon laser-scanning microscope. The cytoplasmic calcium indicator GCaMP6f was expressed selectively in the respective Kenyon cell populations to measure odour-evoked calcium responses. Co-expressed nuclear-localised mCherry (NLS-mCherry) provided a structural reference for image registration, motion correction, and cell segmentation. The imaging data were processed using the analysis code included in this deposition. Further information on the fly genotypes, imaging parameters, odour-stimulation protocol, file organisation, processing workflow, and software requirements is provided in the associated publication and the accompanying 0README.md file.