Raw fastq file of Oxford Nanopore sequencing of whole genomic DNA of citrus red mite (Panonychus citri)

High molecular weight (HMW) DNA was extracted using salt method from about 300 pooled individuals with mixed sex and different development stages. These individuals were firstly transferred into 1.5 ml microtube. We then added 400 μL lysis buffer from the MagAttract HMW DNA Kit (Qiagen, 67563) and 40 μL of 10 μg/mL proteinase K to lysis these individuals. The mixture was incubated overnight (~18h) at 56°C. Then 50 μL of 5 M NaCl was added into the lysed, the mixture was centrifuged at 5000 ×g at 4°C for 15 min. The supernatant was transferred into a new microtube with 400 μL of 100% EtOH and 5 μL of glycogen (20 mg/mL) and cooled at −80°C for 20 min. After centrifuging at 6250 ×g, 4°C for 5 min, the supernatant was removed. The pellet was washed with 1 mL of cold 70% ethanol, centrifuged, and air dried 5 min. Finally, the DNA was resuspended in molecular biology grade H2O. DNA quantitation was used Qubit 3 Fluorometer (Thermo Fisher Scientific, Q32850). Agilent 4200 TapeStation (Agilent, 5067–5365) was used for quality-controlled. The high molecular DNA was used to constructed Oxford Nanopore sequencing library following the manufacturer's instructions (Oxford Nanopore Technology). The constructed DNA library was then sequenced on MINION platform. Guppy (version 6.5.7) was employed to perform base calling with model "dna_r10.4_e8.1_hac".