RNA-Seq of p57Kip2 wild type and p57Kip2 knockout sorted Hopx-GFP+ intestinal stem cells

The goal was to identify the changes in transcriptome profiling (RNA-Seq) caused by p57(CDKN1C) knockout in a population of Hopx+ cells sorted from dissociated intestinal crypts of E18.5 murine embryos Hopx+ cells were sorted from 5 p57 wild type and 5 p57 knockout E18.5 intestines本研究旨在鉴定从18.5天龄小鼠胚胎解离的肠道隐窝中分选得到的Hopx阳性细胞（Hopx+ cells）群体中，由p57（CDKN1C）敲除所引发的转录组测序（RNA-Seq）的差异变化。实验从5例p57野生型和5例p57敲除的18.5天龄小鼠肠道中分选得到上述Hopx阳性细胞。