S1 File - Anoxic Biodegradation of Isosaccharinic Acids at Alkaline pH by Natural Microbial Communities

Fig A: Alpha (L) and beta (R) conformations of isosaccharinic acid; Fig B: High performance anion exchange chromatography trace of CDP liquor.; Fig C: Total ISA concentration in control reactors amended with 50mgml-1 chloramphenicol; Fig D: Bradford assayed protein levels across all three systems.; Fig E: Total carbohydrate assay; Fig F: ISA and ATP assays of pH11 microcosm. Measured ISA concentration and relative light units (RLU) in first 12 weeks of sampling. Modelled ISA concentration shown for comparison.; Table A: Eubacterial clone libraries of pH 7.5, 9.5 and 10 microcosms, with the closest sequence match within the MEGAblast database; Table B Archaeal clone libraries of pH 7.5, 9.5 and 10 microcosms, with the closest sequence match within the MEGAblast database. (DOCX)