Expression data from Ricin-A expressing Drosophila wing discs

Coordinated tissue growth is essential for generation of functionally integrated organs during animal development as well as for tissue homeostasis during adult life. Using Drosophila wing as a model system we have demonstrated that impairing growth, by expression of a cold sensitive version of type 2 ribosome-inactivating protein Ricin-A (RA), within defined territories along the wing primordium triggers a Dmp53-dependent reduction of growth in adjacent non-perturbed cells. To identify novel genes and pathways involved in coordinating intra-organ growth we analysed differential gene expression between wing discs expressing RA and control wing discs expressing GFP in the posterior compartment by using engrailed-Gal4. Three independent RNA samples were prepared from wing imaginal discs of late L3 larvae expressing GFP or GFP plus RA under the control of en-gal4. After dissection of wing discs, the posterior GFP-positive compartment was separated under a fluorescence stereoscope and used for RNA extraction and hybridization on Affymetrix microarrays.