File S1 - Mutant MMP-9 and HGF Gene Transfer Enhance Resolution of CCl4-Induced Liver Fibrosis in Rats: Role of ASH1 and EZH2 Methyltransferases Repression

Supporting information. Table S1, primer sequence and amplicon size. Figure S1, Adenovirus E4 gene detection. Representative gel shows E4 PCR product (714 bp) in the liver at day 7 after second injection of vectors encoding HGF, HGF+ mMMP-9 and mMMP-9. No E4 product was produced in the saline-treated fibrotic group. Figure S2, MT stained livers. Representative photomicrographs of MT-stained liver sections showing A, normal liver with no fibrosis (fibrosis score of 0); B, liver with fibrous expansion of the portal areas (fibrosis score of 1); C, liver with marked septal fibrosis (fibrosis score of 2); D, liver with portal-portal septa, bridging fibrosis but intact architecture (fibrosis score of 3); E, liver with bridging fibrosis with nodules; advanced fibrosis (fibrosis score of 4). (DOCX)