Transcriptome in cultured pericytes after treatment with LDL cholesterol

Immortalized human primary brain vascular pericytes (HBPC; clone ci37) were cultured at 33 °C with 5% CO2/ 95% air in pericyte medium (Catalog number: 1201; Sciencell Research Laboratories, Carlsbad, CA, USA) containing 2% (v/v) fetal bovine serum, 1% (w/v) pericyte growth factors, and penicillin-streptomycin. Culture flasks and plates were coated with rat tail collagen type I (Catalog number: A1048301; Thermo Fisher Scientific, Darmstadt, Germany). To investigate the effects of LDL cholesterol, pericytes were cultured in 24-well plate at 2.0 × 105 cells/well. Before experiments, the culture medium was replaced with serum-free pericyte medium and cells were cultured at 37°C for 3 days to facilitate the cell differentiation. Thereafter, pericytes were treated with purified LDL from human plasma (Catalog number: SAE0053; Sigma-Aldrich Chemie GmbH, Munich, Germany) at 0 or 2 mg/ml for 24 hours. After removal of culture medium, the pericytes on the plate were immediately lysed in RTL Plus buffer for RNA isolation using RNeasy Plus Mini Kit (Qiagen, Hilden, Germany). Thereafter, RNAseq libraries were prepared, sequenced and analyzed.The deposited file contains FPKM results for all annotated genes. Samples “Ct” and “LDL” correspond to treatments with LDL at 0 and 2 mg/mL, respectively.