Z. Xu, H. Ogawa, P. Vagnarelli, J.H. Bergmann, D.F. Hudson, S. Ruchard, T. Fukagawa, W.C. Earnshaw, K. Samejima (2011) CIL:12167, Gallus gallus gallus, memory B cell. CIL. Dataset

Cell of the DT40 chicken B lymphocyte line at mitosis. Z-series of widefield immunofluorescence images captured using a 100x/1.4NA planapo objective lens on a Deltavision system and deconvolved with a constrained iterative algorithm (Applied Precision). Displayed is the central z-slice. Aurora B localizes to centromeres in early mitosis, but fails to transfer to the spindle at anaphase in wild type clone 18 cells grown in the Aurora B inhibitor ZM447439. Cells were stained for Aurora B (green), plus alpha-tubulin (red) and DAPI for DNA (blue). See Fig 6E panel 5 in Xu et al. 2009 J Cell Biol 187, 637-653.

DT40鸡B淋巴细胞系细胞在有丝分裂阶段的细胞图像Z序列。使用Deltavision系统上的100x/1.4NA计划阿波罗物镜进行宽场免疫荧光成像，并通过约束迭代算法（Applied Precision）进行去卷积。展示的是中央Z切片。Aurora B在早期有丝分裂中定位于着丝粒，但在野生型克隆18细胞中，使用Aurora B抑制剂ZM447439培养的细胞在后期无法转移至纺锤体。细胞被染色以检测Aurora B（绿色），以及α-微管蛋白（红色）和DAPI用于DNA（蓝色）。请参阅Xu等人于2009年发表在《细胞生物学杂志》（J Cell Biol）第187卷的第637-653页的第6E图面板5。