Gene expression profile at single cell level of iPSC-derived and primary thymic epithelial cells (TECs)

We used single cell RNA sequencing (scRNA-seq) to explore the development of heterogeneous TEC-like populations from iPSCs. iPSC-derived cells from the stages of anterior foregut endoderm up to mature TEC were collected for scRNA-seq in a timecourse series from day 7 to 133. For days 38 and 81, samples from two separate inductions are provided. To compare the similarity of induced TECs to primary TECs, thymic stromal cells were isolated from three donors and used as controls. One sample of co-cultured primary thymocytes and induced TECs is also included to examine the effects of thymocytes on TEC maturation.