Gene Expression of Double Positive LCK:Hdac3 KO 3w old mice

Analysis of gene expression in double positive (DP) thymoctes from 3w old LCK Hdac3 conditional KO mice. In this data set we sorted GFP double positive (DP) thymocytes from 3w old mice to identify possible signaling and transcripitonal programs that might explain the DP to single positive block in cell maturation Total RNA was pooled from 2 WT and 2 LCK:Hdac3 KO mice for a total of 3 biological replicates.本数据集针对3周龄LCK特异性条件性敲除Hdac3的小鼠的双阳性(DP)胸腺细胞(double positive (DP) thymocytes)开展基因表达分析。本研究通过分选3周龄小鼠的GFP双阳性(DP)胸腺细胞，旨在鉴定可解释细胞成熟过程中双阳性向单阳性发育阻滞的信号通路与转录调控程序。实验中将2只野生型(WT)小鼠与2只LCK:Hdac3条件性敲除小鼠的总RNA(Total RNA)混合，共设置3个生物学重复(biological replicates)。