File S1 - Comprehensive Evaluation and Optimization of Amplicon Library Preparation Methods for High-Throughput Antibody Sequencing

File S1 includes the following: Figure S1. Amplicon libraries on 1% agarose gel. Figure S2. DA duplicates (DA1/DA2, technical replicates) yield highly comparable HTS datasets. Figure S3. Pairwise comparison of HTS datasets from antibody repertoire libraries prepared using the DA and PE method. Figure S4. Pairwise comparison of HTS datasets from antibody repertoire libraries prepared using the ligation and DA method. Figure S5. Pairwise comparison of HTS datasets from antibody repertoire libraries using the ligation and PE method. Figure S6. Variations in the PE protocol have minimal effects on HTS datasets. Figure S7. Comparison of HTS datasets from antibody repertoire libraries prepared using the PE method and lowering the amounts of total RNA input (500–1 ng). Figure S8. VDJ primer trimming reduces VDJ diversity. Table S1. RNA titration (500–5 ng) using the PE method. Table S2. The 30 highest ranked CDR3 amino acid sequences of all RNA titration datasets are shown in a color-coded manner with respective frequencies. Table S3. List of all primers used for ligation, DA, and PE. (DOCX)